Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA.
نویسندگان
چکیده
منابع مشابه
Evaluation of the Ribosomal Protein S1 Gene (rpsA) as a Novel Biomarker for Mycobacterium Species Identification
OBJECTIVES To evaluate the resolution and reliability of the rpsA gene, encoding ribosomal protein S1, as a novel biomarker for mycobacteria species identification. METHODS A segment of the rpsA gene (565 bp) was amplified by PCR from 42 mycobacterial reference strains, 172 nontuberculosis mycobacteria clinical isolates, and 16 M. tuberculosis complex clinical isolates. The PCR products were ...
متن کاملRibosomal protein S1 of Escherichia coli is the effector for the regulation of its own synthesis.
To facilitate the study of the regulation of the rpsA gene, a translational fusion between the rpsA gene and the lacZ gene was constructed. Synthesis of the fusion protein was repressed about 10-fold when rpsA was supplied in trans on a multicopy plasmid. This repression is similar to the post-transcriptional regulation previously found for the wild type rpsA gene. Addition of purified protein ...
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Ribosomes were isolated from E. coli, strain P, as previously described [S] . After extraction of ribosomal proteins by acetic acid protein S6 was separated from the other proteins by a two step elution procedure followed by a gradient elution on DEAE-cellulose. This resulted in several forms of S6 as described below and elsewhere in detail [4]. Amino acid analyses were done on a Durrum D-500 a...
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The complete structure of the Escherichia coli S10 ribosomal protein operon is presented. Based on the DNA sequence, the deduced order of the 11 genes in the operon is rpsJ, rplC, rplD, rplW, rplB, rpsS, rplV, rpsC, rplP, rpmC, rpsQ. The estimated transcribed length of the operon is 5181 base pairs. Putative sequences involved in ribosome binding are discussed. The DNA sequence data corrects se...
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The structural genes for Escherichia coli ribosomal protein (r-protein) genes L1, S4, and S11 were inserted into a plasmid vector containing the lac operator and promoter such that the synthesis of L1, S4, and S11 was controlled by lac regulatory elements. Synthesis of L1, S4, and S11 was stimulated by addition of an inducer of the lac operon (isopropyl thiogalactoside) to exponentially growing...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1982
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.79.4.1008